Return to site

1st Kilobaser Tutorial Video: Workflow

How to make DNA yourself using Kilobaser?

Kilobaser is a lab device that can produce DNA primers and oligos in just 60 minutes on your lab bench. Kilobaser incorporates a microfluidic and a novel reagent cartridge technology. Currently it can synthesize high-quality and ready-to-use DNA primers and oligos. This allows you to do several DNA based experiments in a single day.

Our goal is that scientists are able to make DNA sequences by themselves without being dependent on other service providers. Additionally to that, Kilobaser keeps your data completely private, as your data stays in house. In the video, we will explain to you how easy it is to make your own DNA primers or DNA oligos.

All you have to do is, enter your desired DNA sequence on the tablet screen. Press "add DNA" and then "Start Synthesis". Next the screen tells you every single step you should do using descriptive words and pictures. As you will see in the video it is extremely easy to use, everybody in the lab is able to synthesize oligos by themselves without any special training.
 

On the button it says open lid. You need to press confirm, so the lid opens.

The reagent cartridge is already in the machine and holds material for about 200 bases. So, for every couple primers you synthesize you have to change the cartridge.

Once the lid is open it tells you to remove the placeholder and insert the fluidic chip instead. You have to remove the black placeholder and put the transparent fluidc chip into the machine.
 

After pressing confirm, it tells you to insert the PCR vial. The vial is a standard 0,2 mL PCR vial. The DNA gets flushed into this vial when the synthesis is finished. When the vial is put into Kilobaser, you need to press confirm, again. After that the lid of Kilobaser closes and the synthesis starts. The synthesis is fully automated and in the end you get your desired primer dry and ready-to-use in the PCR vial. The total operation time is about two minutes.
 

That's all we got to do. The synthesis is fully automated. It takes about one hour to synthesize one DNA primer with 20 bases. In the end you have your desired primer dry and ready-to-use in the PCR vial. Remove the vial with your DNA, store it in the fridge or immediately use it. Remove the single use chip and insert a new chip for the next synthesis or put back the placeholder if you want to give Kilobaser a little break before the next synthesis.
 

Our CEO, Alex, shows you how easy and straightforward it gets to make DNA yourself in the video above.

VIDEO TRANSCRIPT
 

Welcome to our first Kilobaser tutorial video. My name is Alex I'm the CEO of the company Kilobaser and this is our first product Kilobaser. Today I'm going to show you how easy it is to make your own DNA primers or DNA oligos. All you have to do is to enter your desire DNA sequence here on the screen. Press add DNA. It will show some data and then you press start. The screen tells you what you should do and shows pictures- So, it is extremely easy. On the button it says open lid. You need to press confirm, so the lid opens. For our system we have two disposables. On the one hand the reagent cartridge. The cartridge is already in the machine and that's material for about 200 bases. So, for every couple primers you synthesize you have to change the cartridge which is really a simple process. So once the lid is open it tells you to remove placeholder and insert the chip. So I remove the black placeholder. This is the microfluidic chip we developed. You need to put this chip into the machine. Press confirm. It tells you to insert the vial. It's a standard PCR vial. The DNA gets actually flashed into this vial when the synthesis is finished. Place it in here. We press again confirm. And it closes lid. So that's all we got to do. The rest is done automatically. It takes about one hour to synthesize primer with 20 bases. The next screen shows you how long you have to wait until the primer is finished.

All Posts
×

Almost done…

We just sent you an email. Please click the link in the email to confirm your subscription!

OK