What is Kilobaser?
Kilobaser is the only device combining microfluidic chip technology and a unique cartridge system. Kilobaser is focused on custom oligos and primers. This is where fast turn-around times are needed. Primers are mainly used for PCR applications and usually about 25 bases long. A PCR system cannot work efficiently and progressively without instant primer supply, therefore Kilobaser is the perfect companion device to accelerate your PCR workflow.
What is PCR?
The polymerase chain reaction is a technique used in medical and biological research labs to make millions of copies of a particular DNA section, from a very small amount of DNA. This is the point where Kilobaser comes into play, because the small amount of DNA you need for the PCR is produced by our device. The PCR is used for DNA sequencing, for the detection of genes in order to identify pathogens during infection and to generate forensic DNA profiles.
Is Kilobaser a DNA sequencer?
No. DNA sequencing is a process that "reads" the precise order of nucleotides within a physical DNA molecule. However, Kilobaser is made for the DNA "writing" process. Kilobaser is a synthesizer that produces the DNA molecule from a given sequence information, which again can be sequenced subsequently.
What is chip based DNA synthesis? Does Kilobaser use it?
Chip based means using a small synthetic chip instead of a reagent carrying tubing system, which wastes a big amount of chemicals during operation. This technique is one of the main reasons why Kilobaser saves costs on a large scale. On the one hand you need a lot less reagents and on the other hand you produce a lot less hazardous waste, manufacturing the same amount of DNA as conventional synthesizers.
What are the benefits of using Kilobaser?
Faster innovation cycles due to quickly available oligos. Lower possible prices for oligos due to lower reagent usage. The customer stays in control of proprietary information.
What are the benefits of Kilobaser in comparison to the current situation?
Chip based means using a small synthetic chip instead of a reagent carrying tube system, that consumes a big amount of chemicals during operation. This technique is one of the main reasons why Kilobaser saves costs on a large scale. On the one hand you need a lot less reagents and on the other hand you produce a lot less hazardous waste.
How pure are the samples and how do you control quality?
The samples after the synthesis still contain salts and some unreacted amidites. The quality of primers with <50 bases is good enough to be used right away without further purification (for example for PCR). An integrated in-line sensor allows you to monitor the product quality. If purification is necessary for a specific application, it can be done by the customer using a purification kit that we will offer. Longer oligos should be purified and tested using sequencing.