DNA Synthesizers Listed By Total Yield

The performance of DNA synthesizers in terms of their total yield varies depending on design and technology. This is for the simple reason that different lab applications consume different amounts of DNA. The list of the 10 most popular DNA synthesizers and service providers should help you to find the best fitting DNA synthesizer for your needs in terms of total yield. The data is based on the information provided by the manufacturers.

A list of the 10 most popular DNA synthesizers

Company	Synthesizer	Yield (mol)	Technology
Twist	(Service provider)	0.2 fmol	Array based
Kilobaser	Kilobaser	300 pmol	Microfluidic chip based
DNA Script	Syntax	300 pmol	Solid support + iDNA
IDT DNA	(Service provider)	25 nmol – 10 µmol	Classic column based
OligoMaker Aps	OligoMaker series	10 nmol – 1 µmol	Classic column based
Polygen	Polygen series	10 nmol – 5 µmol	Classic column based
K&A Laborgeraete	H series	n/a – 40 µmol	Classic column based
Biolytic	Dr. Oligo series	2 nmol – 5 µmol	Classic column based
Cytiva	Oligopilot series	250 µmol – 1,8 mol	Classic column based
CsBio	CS series	200 µmol – 4 mol	Classic column based

What is the limiting factor for the total yield?

In solid phase synthesis, all reagents pass through a column packed with solid phase. On the column surface, there are linkers on which a DNA strand grows during the synthesis.
The more surface area available, the more linkers, and at the same time DNA strands, have room for synthesis. So it is the column size, or surface area, that is the limiting factor.

However, a large column requires a large amount of synthesis chemicals. Some synthesizers allow the user to adjust the column size, but only within narrow limits. The dead volume of a system always remains the same and thus cannot be reduced by a smaller column. The maximum possible reagent flow rate of a synthesizer limits the column size upwards.

Learn about the technology of Kilobaser microfluidic chip based DNA & RNA synthesizer.

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