• TUTORIAL VIDEOS

    Tutorial 1: Basic Workflow

    Tutorial 4: Set-up Kilobaser

    Tutorial 2: Reagent cartridge change

    Tutorial 3: Gas supply

  • FAQ

    Find the right answer for frequently asked questions

    What is Kilobaser?

    What is PCR?

    What is chip based DNA synthesis? Does Kilobaser use it?

    What are the benefits of using Kilobaser?

    What are the benefits of Kilobaser in comparison to the current situation?

    Can the Kilobaser sit on a benchtop and are there any special spatial requirements?

    If the instrument is off, how long does it take to start up and be ready for synthesis?

    What kind of maintenance is involved?

    How much are cartridges?

    What is the shelf life and storage conditions of reagent cartridges/fluidic chips?

    How many oligos can be synthesized with one reagent cartridge?

    How much waste is generated per run?

    How often can you use one fluidic chip?

    Are there any additional purification steps needed after synthesizing oligos?

    Is post-processing included in the instrument?

    How is the quality of the oligos?

    Is the product dried? And how long are oligos stable at room temperature?

    Is it possible to synthesize modified oligos/probes with Kilobaser?

    Can Kilobaser synthesize RNA as well?

  • What is Kilobaser?

    Kilobaser is a desktop DNA synthesizer, which can produce DNA sequences within minutes. It is the only device combining microfluidic chip technology and a unique cartridge system. Kilobaser is focused on custom oligos and primers. This is where fast turn-around times (TAT) are needed. Primers are mainly used for PCR applications and usually about 25 bases long. A PCR system cannot work efficiently and progressively without instant primer supply, therefore Kilobaser is the perfect companion device to accelerate your PCR workflow.

    What is PCR?

    The polymerase chain reaction is a technique used in medical and biological research labs to make millions of copies of a particular DNA section, from a very small amount of DNA. This is the point where Kilobaser comes into play, because the small amount of DNA you need for the PCR is produced by our device. The PCR is used for DNA sequencing, for the detection of genes in order to identify pathogens during infection and to generate forensic DNA profiles.

    What is chip based DNA synthesis? Does Kilobaser use it?

    Chip based means using a small synthetic chip instead of a reagent carrying tubing system, which wastes a big amount of chemicals during operation. This technique is one of the main reasons why Kilobaser saves costs on a large scale. On the one hand you need a lot less reagents and on the other hand you produce a lot less hazardous waste, manufacturing the same amount of DNA as conventional synthesizers.

    What are the benefits of using Kilobaser?

    Faster innovation cycles due to quickly available oligos. Lower possible prices for oligos due to lower reagent usage. The customer stays in control of proprietary information.

    Can the Kilobaser sit on a benchtop and are there any special spatial requirements?

    Yes, Kilobaser can be operated on a benchtop. It should be a stable, flat and dry environment. Behind the device, there should be at least 15 cm of clearance for proper ventilation and necessary electrical and gas connections as well as at least 25 cm of clearance above to give the lid proper clearance to open. The Kilobaser should be operated in normal indoor laboratory conditions. The area must be clean and well-ventilated. Temperature should be stable, and humidity should not be too high as the DNA synthesis is moisture sensitive.

    If the instrument is off, how long does it take to start up and be ready for synthesis?

    If a reagent cartridge is in place from a prior synthesis, roughly one minute. You enter your desired DNA sequence in the software, insert a fresh fluidic chip and a fresh 200µL PCR vial, and then you select “Start Synthesis”.

    Inserting a new reagent cartridge adds 20 minutes prior to the synthesis itself to automatically prepare the new phosphonamidites.

    What kind of maintenance is involved?

    There is no maintenance needed for Kilobaser.

    How much are cartridges?

    One cartridge which is holding all reagents needed for the synthesis of 200 bases is 100 €. Please check our section SHOP for all prices.

    What is the shelf life and storage conditions of reagent cartridges/fluidic chips?

    The shelf life of cartridges is one year minimum, and we recommend reagent cartridges be stored at 4°C. After inserted into Kilobaser, the reagent cartridges can be used for up to two weeks or until 200 bases are synthesized. The cartridge should remain inserted into Kilobaser until it is used up.

    The fluidic chips should be stored in the dark at room temperature.

    How many oligos can be synthesized with one reagent cartridge?

    One reagent cartridge holds material for 200 bases. For example you can print:

    1. Six oligos with 30 bases
    2. Eight oligos with 25 bases
    3. Ten oligos with 20 bases
    4. Two 80mer oligos and two 20mer oligos

    until 200 bases are used up. Basically, you can print oligos with different length until the reagents for 200 bases are finished.

    How much waste is generated per run?

    The total liquid volume in each cartridge is just roughly 18mL. The volume of all microfluidic channels in the microfluidic chip is 4µl and thus Kilobaser produces 50% less hazardous waste than usual column-based synthesizers. Per base, the Kilobaser needs 75µL of total liquid volume which will eventually turn to waste. The waste is constantly pumped back into a waste bottle in the cartridge and can be long term stored in there.

    Since the cartridge contains hazardous chemicals, please ensure cartridges are removed in accordance with current regulations in your country.

    How often can you use one fluidic chip?

    A fluidic chip is a single use chip.

    Are there any additional purification steps needed after synthesizing oligos?

    The primers are eluted and ready-to-use out of Kilobaser for most applications such as PCR/qPCR. An automatic final deprotection/cleavage step is performed by the device after each DNA synthesis.

    There is no de-salting step involved. Due to the very low total volume of all microfluidic channels, our system produces very low concentrations of salt. So far, we haven't encountered any applications where that is an issue.

     

    For highly demanding applications sensitive to even trace amounts of salts, e.g., NMR or crystallography, we recommend using a size exclusion column as a purification step. The columns are available on request. Other specific applications might need HPLC or PAGE purification.

    Is post-processing included in the instrument?

    The DNA comes out of the machine dry and ready to use and automatic cleavage and final deprotection is performed by Kilobaser. There is no de-salting step involved as our system produces an extremely low amount of salt due to the low total reagent volume used while synthesizing.

    How is the quality of the oligos?

    For PCR and many other applications, you can expect the same performance as with primers from online synthesis providers. Oligos printed by Kilobaser show a stepwise yield of 99,5%, which is comparable to any other manufacturer. Have a look at our DNA Synthesis Report.

    Is the product dried? And how long are oligos stable at room temperature?

    The product is a dry DNA of any desired length up to 80 bases.

    Oligos can either be stored dry or resuspended in TE (Tris-EDTA) buffer. Tris-EDTA is a commonly used buffer solution for storing nucleic acids, especially DNA. The buffer keeps the DNA soluble in water and EDTA, a chelator of cations such as magnesium, protects DNA against enzymatic degradation.

    Oligos that are stored at RT or 4°C in TE buffer are more stable than dry oligos. Oligos resuspended in water are the least stable. However, at 4°C, DNA is stable for at least one year under all these conditions. Additionally, it is recommended to store oligos in the dark.

    Is it possible to synthesize modified oligos/probes with Kilobaser?

    Currently Kilobaser synthesizes standard primers only. Reagent cartridges, holding reagents for probes and RNA synthesis are under development and will be available as consumables for Kilobaser in the end of 2020.

    Can Kilobaser synthesize RNA as well?

    Currently, DNA only. We have started development on RNA reagent cartridges this year and will be available mid next year. In general, the Kilobaser hardware is able to synthesize both DNA and RNA, means RNA can be synthesized with the same device but will require another cartridge for RNA synthesis.

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